Single-concentration broth microdilution test for detection of high-level aminoglycoside resistance in enterococci
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Single-concentration broth microdilution test for detection of high-level aminoglycoside resistance in enterococci.
Growth in a single broth microdilution well containing gentamicin at a concentration of 500 micrograms/ml predicted high-level resistance to gentamicin (MIC, greater than or equal to 2,000 micrograms/ml) in 505 of 508 clinical isolates of enterococci. Failure to achieve synergistic killing with the combination of penicillin and an aminoglycoside was demonstrated with 100% specificity in 20 stra...
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cultures of 45 previously characterized enterococci (17 E. faecalis, 16 E. faecium and 12 E. spp) were included in the study. All isolates were tested in the Phoenix ™ Automated Microbiology System at an approximate inoculum concentration of 5x10 5 cfu per mL in a cation supplemented Mueller-Hinton broth. Phoenix ™ results were compared to the results with both broth and agar screening tests. T...
متن کاملMultiplex PCR for detection of aminoglycoside resistance genes in enterococci.
A multiplex PCR procedure for detecting the aminoglycoside resistance genes aac(6')-Ie-aph(2")-Ia, aph(2")-Ib, aph(2")-Ic, aph(2")-Id, aph(3')-IIIa, and ant(4')-Ia was evaluated and found to determine accurately the presence of these genes in enterococci.
متن کاملComparison of microscan broth microdilution, synergy quad plate agar dilution, and disk diffusion screening methods for detection of high-level aminoglycoside resistance in enterococcus species.
We compared the dried MicroScan microdilution panel, Synergy Quad plate agar dilution, and high-potency disk diffusion screening methods for the detection of high-level aminoglycoside resistance in 815 enterococcal bloodstream isolates. Agreement between the three methods was 99% when testing for high-level gentamicin resistance and 96% when testing for high-level streptomycin resistance.
متن کاملMultilaboratory evaluation of screening methods for detection of high-level aminoglycoside resistance in enterococci. National Committee for Clinical Laboratory Standards Study Group on Enterococci.
Since the early 1970s, the synergistic activity of an aminoglycoside with a cell wall-active agent has been predicted by determining the ability of an enterococcus to grow in the presence of high levels of the aminoglycoside (usually > or = 2,000 micrograms/ml). However, a variety of media and concentrations of aminoglycosides has been used for this screening procedure. In the present study, we...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1987
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.25.12.2443-2444.1987